1.首要(yao)原则：细(xi)(xi)(xi)胞(bao)不重(zhong)要(yao)情况下(xia)立即(ji)丢弃，培(pei)养(yang)(yang)(yang)箱灭(mie)菌，所(suo)用(yong)(yong)培(pei)养(yang)(yang)(yang)基(ji)也都(dou)(dou)(dou)(dou)要(yao)丢弃，器械(xie)等重(zhong)新灭(mie)菌或(huo)拆(chai)用(yong)(yong)新的(de)。2.细(xi)(xi)(xi)菌污(wu)染(ran)一(yi)般都(dou)(dou)(dou)(dou)救不回来了，发(fa)现(xian)的(de)时(shi)(shi)(shi)候(hou)培(pei)养(yang)(yang)(yang)基(ji)一(yi)般都(dou)(dou)(dou)(dou)很浑浊且细(xi)(xi)(xi)胞(bao)都(dou)(dou)(dou)(dou)死了3.污(wu)染(ran)且细(xi)(xi)(xi)胞(bao)很重(zhong)要(yao)时(shi)(shi)(shi)：遇(yu)到念球菌污(wu)染(ran)，且细(xi)(xi)(xi)胞(bao)为基(ji)因改造细(xi)(xi)(xi)胞(bao)，非常(chang)重(zhong)要(yao)。如231贴壁(bi)乳(ru)腺(xian)细(xi)(xi)(xi)胞(bao)，发(fa)现(xian)细(xi)(xi)(xi)胞(bao)周围出现(xian)很小的(de)串珠透亮圆(yuan)点，非常(chang)像念球菌污(wu)染(ran)，此(ci)时(shi)(shi)(shi)细(xi)(xi)(xi)胞(bao)状态(tai)尚可，且污(wu)染(ran)少。处理如下(xia)：用(yong)(yong)预热或(huo)室(shi)温PBS清(qing)洗(xi)(xi)3次(ci)，可适当振(zhen)摇，将(jiang)污(wu)染(ran)冲洗(xi)(xi)下(xia)来。随(sui)后(hou)(hou)(hou)加入10-20%双抗到培(pei)养(yang)(yang)(yang)瓶，置(zhi)于37度培(pei)养(yang)(yang)(yang)箱1h，之(zhi)后(hou)(hou)(hou)再用(yong)(yong)PBS清(qing)洗(xi)(xi)三遍(bian)，直至视野下(xia)无可见污(wu)染(ran)。此(ci)时(shi)(shi)(shi)细(xi)(xi)(xi)胞(bao)也被冲下(xia)大部分(fen)，因此(ci)此(ci)方法只适用(yong)(yong)在细(xi)(xi)(xi)胞(bao)贴壁(bi)强，状态(tai)好，密度高时(shi)(shi)(shi)使用(yong)(yong)。之(zhi)后(hou)(hou)(hou)每(mei)天再更换培(pei)养(yang)(yang)(yang)基(ji)，每(mei)次(ci)用(yong)(yong)PBS冲洗(xi)(xi)2遍(bian)。过几天细(xi)(xi)(xi)胞(bao)状态(tai)尚可时(shi)(shi)(shi)，消化离心时(shi)(shi)(shi)用(yong)(yong)500r，3min，去掉(diao)上清(qing)，重(zhong)复3次(ci)。这个方法是(shi)根据文(wen)献可利用(yong)(yong)念球菌和细(xi)(xi)(xi)胞(bao)体积重(zhong)量差异(yi)实现(xian)分(fen)离。基(ji)本(ben)上这一(yi)步做完(wan)以后(hou)(hou)(hou)，污(wu)染(ran)就基(ji)本(ben)了，接下(xia)来就注意(yi)多(duo)观察，勤换液就行。细(xi)(xi)(xi)胞(bao)增殖与细(xi)(xi)(xi)胞(bao)凋(diao)亡、细(xi)(xi)(xi)胞(bao)周期等是(shi)研究(jiu)(jiu)的(de)重(zhong)要(yao)表型，是(shi)分(fen)子生物学和药理学研究(jiu)(jiu)解决(jue)的(de)问题(ti)之(zhi)一(yi)。广西组(zu)织科研技(ji)术服(fu)务实验室(shi)

转录组测序结果及TCGA数据库分(fen)析）图(tu)5RNA-Seq和m6A-seq联(lian)合鉴(jian)定(ding)SOCS2是介导的(de)(de)(de)(de)m6A修(xiu)饰(shi)的(de)(de)(de)(de)下游(you)靶(ba)基(ji)(ji)(ji)因PLoSOne2015,在(zai)许多不同(tong)种(zhong)类的(de)(de)(de)(de)RNA中，都已观察到N6-腺(xian)苷(m6A)的(de)(de)(de)(de)甲(jia)(jia)(jia)基(ji)(ji)(ji)化(hua)(hua)，但其在(zai)microRNAs中还(hai)没(mei)有被(bei)研(yan)究。研(yan)究者在(zai)FTO1C1,FTO2D4和FTO3C3细胞系(xi)中，通(tong)过敲除(chu)m6A甲(jia)(jia)(jia)基(ji)(ji)(ji)转移(yi)酶(mei)FTO筛(shai)选到表达差异的(de)(de)(de)(de)microRNA,说明miRNA受m6A甲(jia)(jia)(jia)基(ji)(ji)(ji)化(hua)(hua)的(de)(de)(de)(de)调(diao)控。进一(yi)步通(tong)过MeRIP-Seq发(fa)现相(xiang)当(dang)一(yi)部分(fen)的(de)(de)(de)(de)microRNA具有m6A修(xiu)饰(shi)。通(tong)过motif分(fen)析，他们发(fa)现了(le)区分(fen)甲(jia)(jia)(jia)基(ji)(ji)(ji)化(hua)(hua)和非甲(jia)(jia)(jia)基(ji)(ji)(ji)化(hua)(hua)microRNA的(de)(de)(de)(de)一(yi)致序列。该文章所(suo)述的(de)(de)(de)(de)表观遗传修(xiu)饰(shi)在(zai)基(ji)(ji)(ji)因表达的(de)(de)(de)(de)转录调(diao)控的(de)(de)(de)(de)复杂性上(shang)增加了(le)一(yi)个新的(de)(de)(de)(de)层(ceng)次。图(tu)FTO敲除(chu)对甲(jia)(jia)(jia)基(ji)(ji)(ji)化(hua)(hua)的(de)(de)(de)(de)miRNAs的(de)(de)(de)(de)稳定(ding)状态的(de)(de)(de)(de)影响。参考文献(xian)Y,DominissiniD,RechaviG,HeC:Geneexpressionregulationmediatedthroughreversiblem(6)(5):'(1):(12):(6):(1):(1):(uridine)(41):(6)(7540):(1):(7481):(4):(6)A-LAIC-seqrevealsthecensusandcomplexityofthem(6)(8):'UTRm(6)(4):(7544):(6)(6):(5):(7667):(2):"">panstyle="color:#f5c81c;">xiainducesthebreastcancerstemcellphenotypebyHIF-dependentandALKBH5-mediatedm(6)(14):"">panstyle="color:#f5c81c;">(40):(6)(3):(1):(1):(4):(11):。宁夏动物科(ke)研(yan)技术服务分(fen)离(li)干货分(fen)享｜选对实验室常(chang)用培养基(ji)(ji)(ji)DMEM和1640。

应(ying)退回纯酒精中(zhong)重新(xin)脱水(shui)(shui)，然后再(zai)透(tou)(tou)明(ming)(ming)，否(fou)则切(qie)片难(nan)以(yi)镜检(jian)。（4）二甲苯应(ying)尽量保(bao)持无(wu)水(shui)(shui)，应(ying)经(jing)常(chang)更换，或用纱布包无(wu)水(shui)(shui)硫酸(suan)铜(tong)放入(ru)染(ran)(ran)(ran)色(se)(se)缸内吸收(shou)水(shui)(shui)分。5.透(tou)(tou)蜡(la)注(zhu)意事项（1）透(tou)(tou)蜡(la)的(de)目(mu)的(de)是除去组(zu)(zu)织(zhi)中(zhong)的(de)透(tou)(tou)明(ming)(ming)剂(ji)（如二甲苯等），使石蜡(la)渗透(tou)(tou)到组(zu)(zu)织(zhi)内部达到饱和程(cheng)度(du)以(yi)便包埋。透(tou)(tou)蜡(la)时(shi)(shi)间(jian)根(gen)据组(zu)(zu)织(zhi)大(da)小而(er)定。（2）尽量保(bao)持在(zai)较(jiao)(jiao)低(di)(di)(di)温(wen)度(du)中(zhong)进行，以(yi)石蜡(la)不(bu)(bu)凝固(gu)为(wei)度(du)。（3）透(tou)(tou)蜡(la)温(wen)度(du)要恒定，不(bu)(bu)可忽高忽低(di)(di)(di)。（4）操作要迅速，力求在(zai)短(duan)的(de)时(shi)(shi)间(jian)内完成石蜡(la)透(tou)(tou)入(ru)过(guo)(guo)程(cheng)，以(yi)免引(yin)起(qi)组(zu)(zu)织(zhi)变硬、变脆、收(shou)缩等。（5）注(zhu)意温(wen)度(du)不(bu)(bu)要过(guo)(guo)高，以(yi)免组(zu)(zu)织(zhi)发(fa)脆。6.染(ran)(ran)(ran)色(se)(se)水(shui)(shui)洗注(zhu)意事项（1）染(ran)(ran)(ran)色(se)(se)原理：伊(yi)红主要染(ran)(ran)(ran)细(xi)(xi)胞(bao)质(zhi)，着(zhe)色(se)(se)浓(nong)淡(dan)应(ying)与苏木精染(ran)(ran)(ran)细(xi)(xi)胞(bao)核的(de)浓(nong)淡(dan)相配合，如果细(xi)(xi)胞(bao)核染(ran)(ran)(ran)色(se)(se)较(jiao)(jiao)浓(nong)，细(xi)(xi)胞(bao)质(zhi)也(ye)应(ying)浓(nong)染(ran)(ran)(ran)，以(yi)获得(de)鲜明(ming)(ming)的(de)对比。（2）染(ran)(ran)(ran)色(se)(se)时(shi)(shi)间(jian)应(ying)根(gen)据染(ran)(ran)(ran)色(se)(se)剂(ji)的(de)成熟程(cheng)度(du)及室(shi)温(wen)高低(di)(di)(di)，适当缩短(duan)或延长。室(shi)温(wen)高时(shi)(shi)促进染(ran)(ran)(ran)色(se)(se)，染(ran)(ran)(ran)色(se)(se)时(shi)(shi)间(jian)可短(duan)些，否(fou)则可适当延长时(shi)(shi)间(jian)，冬季室(shi)温(wen)低(di)(di)(di)时(shi)(shi)可放入(ru)恒温(wen)箱中(zhong)染(ran)(ran)(ran)色(se)(se)。（3）注(zhu)意流水(shui)(shui)不(bu)(bu)能(neng)过(guo)(guo)大(da)，以(yi)防切(qie)片脱落(luo)。（4）如果细(xi)(xi)胞(bao)核染(ran)(ran)(ran)色(se)(se)较(jiao)(jiao)浅，细(xi)(xi)胞(bao)质(zhi)也(ye)应(ying)淡(dan)染(ran)(ran)(ran)。可在(zai)伊(yi)红乙(yi)醇(chun)液中(zhong)滴(di)加数(shu)滴(di)冰醋酸(suan)助(zhu)染(ran)(ran)(ran)，促使细(xi)(xi)胞(bao)质(zhi)容易着(zhe)色(se)(se)，并且(qie)经(jing)乙(yi)醇(chun)脱水(shui)(shui)时(shi)(shi)不(bu)(bu)易褪(tun)色(se)(se)。

启医疗，个体(ti)化用药贝克曼(man)库(ku)尔特(te)细(xi)胞专(zhuan)题--助力病毒载体(ti)纯化、细(xi)胞特(te)性(xing)分(fen)(fen)析(xi)产品库(ku)仪(yi)(yi)(yi)器(qi)设(she)(she)(she)备耗(hao)材试(shi)剂抗体(ti)技术服务生物(wu)(wu)(wu)芯片(pian)芯片(pian)扫描(miao)仪(yi)(yi)(yi)|芯片(pian)点(dian)样(yang)仪(yi)(yi)(yi)|生物(wu)(wu)(wu)芯片(pian)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|生物(wu)(wu)(wu)芯片(pian)|其(qi)(qi)(qi)它(ta)测(ce)读系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)洗(xi)板机(ji)|多(duo)功(gong)能(neng)(neng)筛选系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|大型(xing)分(fen)(fen)析(xi)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|化学发光(guang)(guang)(guang)(guang)(guang)(guang)(guang)检测(ce)仪(yi)(yi)(yi)|分(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)度计|其(qi)(qi)(qi)它(ta)|光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)原子吸收(shou)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|可见光(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|荧光(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|红(hong)外(wai)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|近红(hong)外(wai)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|LIBS光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|拉曼(man)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|紫外(wai)/可见/近红(hong)外(wai)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)谱(pu)(pu)(pu)(pu)仪(yi)(yi)(yi)|其(qi)(qi)(qi)它(ta)分(fen)(fen)子生物(wu)(wu)(wu)实(shi)验仪(yi)(yi)(yi)器(qi)电泳设(she)(she)(she)备|紫外(wai)设(she)(she)(she)备|普通PCR仪(yi)(yi)(yi)|定量PCR仪(yi)(yi)(yi)|数字PCR仪(yi)(yi)(yi)|DNA/有机(ji)/多(duo)肽(tai)合成|转基(ji)(ji)因仪(yi)(yi)(yi)|其(qi)(qi)(qi)它(ta)显微(wei)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)倒置显微(wei)镜|实(shi)体(ti)显微(wei)镜|生物(wu)(wu)(wu)显微(wei)镜|电子显微(wei)镜其(qi)(qi)(qi)它(ta)显微(wei)镜|显微(wei)操纵(zong)|附(fu)件(jian)(jian)和滤光(guang)(guang)(guang)(guang)(guang)(guang)(guang)片(pian)|其(qi)(qi)(qi)它(ta)色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)液相(xiang)色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|制(zhi)备液相(xiang)色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|毛(mao)细(xi)管LC系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)气(qi)相(xiang)色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|离子色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)检测(ce)器(qi)样(yang)品管理工具|色(se)(se)(se)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)附(fu)件(jian)(jian)质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)飞(fei)行质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)|四(si)极(ji)杆质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)|离子阱质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)|等离子质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)|毛(mao)细(xi)管电泳/质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)联用系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|杂(za)交质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)|质(zhi)(zhi)(zhi)(zhi)谱(pu)(pu)(pu)(pu)标准品|其(qi)(qi)(qi)它(ta)实(shi)验室(shi)自动化氨(an)基(ji)(ji)酸分(fen)(fen)析(xi)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|蛋(dan)(dan)白(bai)纯化系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|蛋(dan)(dan)白(bai)质(zhi)(zhi)(zhi)(zhi)翻(fan)译系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|全(quan)自动分(fen)(fen)液系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|核酸提取纯化全(quan)自动血液采样(yang)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|基(ji)(ji)因组(zu)/蛋(dan)(dan)白(bai)组(zu)设(she)(she)(she)备DNA测(ce)序(xu)仪(yi)(yi)(yi)|全(quan)基(ji)(ji)因组(zu)测(ce)序(xu)仪(yi)(yi)(yi)|基(ji)(ji)因分(fen)(fen)型(xing)系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|双向电泳系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|蛋(dan)(dan)白(bai)质(zhi)(zhi)(zhi)(zhi)纯化|蛋(dan)(dan)白(bai)质(zhi)(zhi)(zhi)(zhi)斑点(dian)切取系(xi)(xi)(xi)(xi)统(tong)(tong)(tong)(tong)|其(qi)(qi)(qi)它(ta)神经生物(wu)(wu)(wu)学仪(yi)(yi)(yi)器(qi)动物(wu)(wu)(wu)功(gong)能(neng)(neng)检测(ce)设(she)(she)(she)备|动物(wu)(wu)(wu)处(chu)理设(she)(she)(she)备|。随着科技的(de)不断进步，科研人(ren)员将能(neng)(neng)够开发出更为精确、实(shi)用的(de)动物(wu)(wu)(wu)模型(xing)，更好地为人(ren)类健康保驾护航。

动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型在(zai)科(ke)(ke)(ke)(ke)(ke)研(yan)中(zhong)(zhong)有着普遍的(de)(de)(de)应(ying)用(yong)。首先，它们可(ke)(ke)(ke)以帮(bang)助科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)深入理(li)解的(de)(de)(de)共同性，即不(bu)同物(wu)(wu)(wu)(wu)(wu)种之(zhi)间存在(zai)的(de)(de)(de)共有理(li)变(bian)化过(guo)(guo)程。通过(guo)(guo)对(dui)动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型的(de)(de)(de)研(yan)究(jiu)(jiu)，科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)可(ke)(ke)(ke)以更(geng)清楚地了解的(de)(de)(de)发(fa)展(zhan)(zhan)过(guo)(guo)程和(he)(he)(he)机制(zhi)，为(wei)人(ren)类的(de)(de)(de)检查提(ti)供(gong)理(li)论(lun)依据(ju)。其(qi)次(ci)，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型还为(wei)新(xin)研(yan)发(fa)和(he)(he)(he)苗测试提(ti)供(gong)了的(de)(de)(de)平台。在(zai)研(yan)发(fa)过(guo)(guo)程中(zhong)(zhong)，科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)可(ke)(ke)(ke)以通过(guo)(guo)对(dui)动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型进行处理(li)，观察(cha)其(qi)和(he)(he)(he)副(fu)作用(yong)，为(wei)新(xin)的(de)(de)(de)临(lin)床试验提(ti)供(gong)依据(ju)。而在(zai)苗测试中(zhong)(zhong)，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型则可(ke)(ke)(ke)以用(yong)来评估苗的(de)(de)(de)性和(he)(he)(he)安全性。此(ci)外，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型还为(wei)科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)提(ti)供(gong)了研(yan)究(jiu)(jiu)人(ren)类的(de)(de)(de)跨学科(ke)(ke)(ke)(ke)(ke)方法(fa)。例如，通过(guo)(guo)比较人(ren)类和(he)(he)(he)动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型的(de)(de)(de)基(ji)因组(zu)学、蛋白(bai)质组(zu)学等数(shu)据(ju)，可(ke)(ke)(ke)以发(fa)现与发(fa)生相(xiang)关的(de)(de)(de)关键基(ji)因和(he)(he)(he)蛋白(bai)质，从而为(wei)的(de)(de)(de)和(he)(he)(he)检查提(ti)供(gong)新(xin)的(de)(de)(de)思(si)路。虽然(ran)动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型在(zai)科(ke)(ke)(ke)(ke)(ke)研(yan)中(zhong)(zhong)发(fa)挥了巨大的(de)(de)(de)作用(yong)，但也(ye)(ye)存在(zai)一些挑(tiao)战(zhan)。首先，由于物(wu)(wu)(wu)(wu)(wu)种差异(yi)的(de)(de)(de)存在(zai)，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型的(de)(de)(de)表现与人(ren)类可(ke)(ke)(ke)能存在(zai)差异(yi)，因此(ci)需要谨慎使(shi)用(yong)。此(ci)外，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型的(de)(de)(de)伦(lun)理(li)问题也(ye)(ye)不(bu)容忽视，科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)需要在(zai)符合伦(lun)理(li)规定的(de)(de)(de)前提(ti)下进行相(xiang)关研(yan)究(jiu)(jiu)。尽管(guan)存在(zai)挑(tiao)战(zhan)，动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型的(de)(de)(de)发(fa)展(zhan)(zhan)前景(jing)仍(reng)然(ran)值(zhi)得期(qi)待。随着科(ke)(ke)(ke)(ke)(ke)技(ji)的(de)(de)(de)不(bu)断进步，科(ke)(ke)(ke)(ke)(ke)研(yan)人(ren)员(yuan)(yuan)(yuan)将能够开发(fa)出更(geng)为(wei)精(jing)确、实(shi)用(yong)的(de)(de)(de)动(dong)(dong)物(wu)(wu)(wu)(wu)(wu)模(mo)(mo)型。可(ke)(ke)(ke)以发(fa)现与疾病(bing)发(fa)生相(xiang)关的(de)(de)(de)关键基(ji)因和(he)(he)(he)蛋白(bai)质，从而为(wei)疾病(bing)的(de)(de)(de)预防和(he)(he)(he)检查提(ti)供(gong)新(xin)的(de)(de)(de)思(si)路。黑(hei)龙江科(ke)(ke)(ke)(ke)(ke)研(yan)技(ji)术服务分(fen)离(li)

动物模型的表(biao)现与人类疾病可(ke)能存(cun)在差(cha)异，因(yin)此需要谨慎使用。广西组织科研技术服(fu)务(wu)实验室

首先，预(yu)实(shi)(shi)(shi)(shi)验(yan)(yan)必须要(yao)(yao)当做正式实(shi)(shi)(shi)(shi)验(yan)(yan)来(lai)对待（态度要(yao)(yao)放端正，这是(shi)必须的(de)(de)）。预(yu)实(shi)(shi)(shi)(shi)验(yan)(yan)的(de)(de)每一(yi)(yi)步都(dou)需(xu)要(yao)(yao)详细规划，多方筹谋。不论是(shi)实(shi)(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)(dong)(dong)物(wu)的(de)(de)选(xuan)择(ze)(ze)，还(hai)是(shi)检(jian)测试(shi)剂的(de)(de)购(gou)买(mai)(mai)，都(dou)尽量和正式实(shi)(shi)(shi)(shi)验(yan)(yan)统(tong)一(yi)(yi)标准。建(jian)议大家先把所有(you)(you)试(shi)剂和购(gou)买(mai)(mai)完毕后，再去购(gou)买(mai)(mai)实(shi)(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)(dong)(dong)物(wu)。因(yin)(yin)为动(dong)(dong)(dong)(dong)物(wu)会(hui)长(zhang)胖，长(zhang)胖后给(ji)剂量就要(yao)(yao)增加，不仅多花(hua)钱，并且(qie)还(hai)容易影响实(shi)(shi)(shi)(shi)验(yan)(yan)效果(guo)。笔(bi)者(zhe)曾经(jing)提(ti)(ti)(ti)(ti)前将(jiang)实(shi)(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)(dong)(dong)物(wu)买(mai)(mai)回，但因(yin)(yin)为特殊原因(yin)(yin)没(mei)能购(gou)买(mai)(mai)到(dao)造(zao)模(mo)(mo)，终只能忍(ren)痛(tong)割(ge)爱将(jiang)动(dong)(dong)(dong)(dong)物(wu)赠送(song)他人，白(bai)白(bai)亏(kui)了(le)一(yi)(yi)笔(bi)血汗钱（那批老鼠在我(wo)这儿白(bai)吃白(bai)喝长(zhang)得(de)太(tai)胖，没(mei)办(ban)法用作(zuo)造(zao)模(mo)(mo)）。动(dong)(dong)(dong)(dong)物(wu)及试(shi)剂购(gou)买(mai)(mai)首先需(xu)要(yao)(yao)考(kao)虑(lv)：实(shi)(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)(dong)(dong)物(wu)品种(zhong)、体(ti)重、性(xing)别、周(zhou)龄（通(tong)常根据(ju)既往文(wen)献报(bao)道可(ke)以获得(de)答案(an)）、数量（需(xu)要(yao)(yao)考(kao)虑(lv)到(dao)实(shi)(shi)(shi)(shi)验(yan)(yan)有(you)(you)一(yi)(yi)定(ding)动(dong)(dong)(dong)(dong)物(wu)死亡率或者(zhe)动(dong)(dong)(dong)(dong)物(wu)本身(shen)会(hui)打架(jia)互殴，因(yin)(yin)此需(xu)要(yao)(yao)提(ti)(ti)(ti)(ti)前购(gou)买(mai)(mai)足够的(de)(de)动(dong)(dong)(dong)(dong)物(wu)）。动(dong)(dong)(dong)(dong)物(wu)购(gou)买(mai)(mai)的(de)(de)途径、安置的(de)(de)地(di)(di)点，饮(yin)食管理（一(yi)(yi)般实(shi)(shi)(shi)(shi)验(yan)(yan)室会(hui)有(you)(you)动(dong)(dong)(dong)(dong)物(wu)房，也可(ke)以从(cong)其(qi)他地(di)(di)方购(gou)买(mai)(mai)），动(dong)(dong)(dong)(dong)物(wu)免证明、伦理证明需(xu)要(yao)(yao)提(ti)(ti)(ti)(ti)前准备好。实(shi)(shi)(shi)(shi)验(yan)(yan)相关的(de)(de)试(shi)剂和：比(bi)如造(zao)模(mo)(mo)和，动(dong)(dong)(dong)(dong)物(wu)干预(yu)所需(xu)，阳性(xing)选(xuan)择(ze)(ze)及购(gou)买(mai)(mai)（有(you)(you)些(xie)购(gou)买(mai)(mai)很困难，必须通(tong)过特殊程(cheng)序才(cai)能买(mai)(mai)到(dao)）。如果(guo)涉及到(dao)有(you)(you)创操(cao)作(zuo)，要(yao)(yao)提(ti)(ti)(ti)(ti)前准备好（建(jian)议提(ti)(ti)(ti)(ti)前购(gou)买(mai)(mai)），手术。需(xu)要(yao)(yao)了(le)解自身(shen)实(shi)(shi)(shi)(shi)验(yan)(yan)平台(tai)能完成哪(na)些(xie)技术。广西(xi)组织科(ke)研技术服务(wu)实(shi)(shi)(shi)(shi)验(yan)(yan)室

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