1.首要原则：细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)重(zhong)要情(qing)况下(xia)立即丢(diu)弃，培(pei)养(yang)箱(xiang)灭菌，所用(yong)培(pei)养(yang)基(ji)也都要丢(diu)弃，器械等(deng)重(zhong)新(xin)灭菌或拆用(yong)新(xin)的(de)(de)(de)。2.细(xi)(xi)(xi)菌污染一般(ban)都救不(bu)回来(lai)了，发(fa)现(xian)的(de)(de)(de)时(shi)(shi)(shi)候培(pei)养(yang)基(ji)一般(ban)都很浑浊且细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)都死了3.污染且细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)很重(zhong)要时(shi)(shi)(shi)：遇到(dao)(dao)念(nian)球菌污染，且细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)为基(ji)因(yin)改造细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，非常重(zhong)要。如(ru)231贴壁(bi)(bi)乳(ru)腺(xian)细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，发(fa)现(xian)细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周围出现(xian)很小(xiao)的(de)(de)(de)串珠透亮圆点，非常像念(nian)球菌污染，此(ci)(ci)时(shi)(shi)(shi)细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)状(zhuang)(zhuang)态(tai)尚可，且污染少。处理如(ru)下(xia)：用(yong)预热或室温PBS清(qing)洗3次，可适(shi)当振摇，将污染冲洗下(xia)来(lai)。随后加(jia)入10-20%双抗到(dao)(dao)培(pei)养(yang)瓶，置于37度培(pei)养(yang)箱(xiang)1h，之(zhi)后再用(yong)PBS清(qing)洗三遍(bian)，直至(zhi)视野下(xia)无可见(jian)污染。此(ci)(ci)时(shi)(shi)(shi)细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)也被冲下(xia)大部分，因(yin)此(ci)(ci)此(ci)(ci)方(fang)法只适(shi)用(yong)在细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)贴壁(bi)(bi)强，状(zhuang)(zhuang)态(tai)好，密度高时(shi)(shi)(shi)使(shi)用(yong)。之(zhi)后每(mei)天再更换(huan)培(pei)养(yang)基(ji)，每(mei)次用(yong)PBS冲洗2遍(bian)。过(guo)几天细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)状(zhuang)(zhuang)态(tai)尚可时(shi)(shi)(shi)，消(xiao)化离(li)(li)心(xin)时(shi)(shi)(shi)用(yong)500r，3min，去(qu)掉上清(qing)，重(zhong)复3次。这个(ge)方(fang)法是(shi)根据文献可利(li)用(yong)念(nian)球菌和(he)细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)体积重(zhong)量(liang)差异实现(xian)分离(li)(li)。基(ji)本上这一步(bu)做完以后，污染就基(ji)本了，接下(xia)来(lai)就注意多观察，勤换(huan)液就行(xing)。细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增殖与细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凋亡、细(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周期等(deng)是(shi)研(yan)究(jiu)的(de)(de)(de)重(zhong)要表型，是(shi)分子生物学和(he)药(yao)理学研(yan)究(jiu)解决的(de)(de)(de)问题之(zhi)一。广西组(zu)织(zhi)科研(yan)技(ji)术服(fu)务实验(yan)室

转(zhuan)录组测序(xu)(xu)结果(guo)及TCGA数(shu)据库分(fen)(fen)析）图(tu)5RNA-Seq和(he)m6A-seq联合(he)鉴定SOCS2是(shi)介导的(de)(de)(de)m6A修饰(shi)的(de)(de)(de)下游靶基(ji)因PLoSOne2015,在(zai)许多不同种类的(de)(de)(de)RNA中(zhong)，都已(yi)观察到(dao)N6-腺苷(m6A)的(de)(de)(de)甲(jia)基(ji)化，但其在(zai)microRNAs中(zhong)还没有(you)被研(yan)究。研(yan)究者在(zai)FTO1C1,FTO2D4和(he)FTO3C3细胞系(xi)中(zhong)，通(tong)过(guo)敲除m6A甲(jia)基(ji)转(zhuan)移酶FTO筛(shai)选(xuan)到(dao)表达差(cha)异(yi)的(de)(de)(de)microRNA,说(shuo)明miRNA受m6A甲(jia)基(ji)化的(de)(de)(de)调控。进一(yi)步通(tong)过(guo)MeRIP-Seq发现(xian)相当一(yi)部(bu)分(fen)(fen)的(de)(de)(de)microRNA具有(you)m6A修饰(shi)。通(tong)过(guo)motif分(fen)(fen)析，他们发现(xian)了(le)区(qu)分(fen)(fen)甲(jia)基(ji)化和(he)非甲(jia)基(ji)化microRNA的(de)(de)(de)一(yi)致序(xu)(xu)列。该文章所(suo)述的(de)(de)(de)表观遗传修饰(shi)在(zai)基(ji)因表达的(de)(de)(de)转(zhuan)录调控的(de)(de)(de)复杂性上(shang)增加了(le)一(yi)个新的(de)(de)(de)层(ceng)次。图(tu)FTO敲除对(dui)甲(jia)基(ji)化的(de)(de)(de)miRNAs的(de)(de)(de)稳定状态的(de)(de)(de)影(ying)响(xiang)。参考文献(xian)Y,DominissiniD,RechaviG,HeC:Geneexpressionregulationmediatedthroughreversiblem(6)(5):'(1):(12):(6):(1):(1):(uridine)(41):(6)(7540):(1):(7481):(4):(6)A-LAIC-seqrevealsthecensusandcomplexityofthem(6)(8):'UTRm(6)(4):(7544):(6)(6):(5):(7667):(2):"">panstyle="color:#f5c81c;">xiainducesthebreastcancerstemcellphenotypebyHIF-dependentandALKBH5-mediatedm(6)(14):"">panstyle="color:#f5c81c;">(40):(6)(3):(1):(1):(4):(11):。宁夏(xia)动物(wu)科研(yan)技(ji)术服(fu)务分(fen)(fen)离(li)干货分(fen)(fen)享(xiang)｜选(xuan)对(dui)实验室常用培养基(ji)DMEM和(he)1640。

应(ying)(ying)退回纯酒精(jing)中(zhong)重新脱(tuo)(tuo)水(shui)，然后再透(tou)明，否则(ze)切(qie)片难以(yi)镜检。（4）二甲苯应(ying)(ying)尽(jin)量(liang)保持无水(shui)，应(ying)(ying)经常更换，或(huo)用纱(sha)布包(bao)无水(shui)硫酸铜放(fang)入染(ran)色(se)(se)(se)缸内吸收(shou)水(shui)分。5.透(tou)蜡(la)注(zhu)(zhu)(zhu)意事项（1）透(tou)蜡(la)的(de)目(mu)的(de)是除去组(zu)织(zhi)(zhi)(zhi)中(zhong)的(de)透(tou)明剂（如二甲苯等），使石蜡(la)渗透(tou)到组(zu)织(zhi)(zhi)(zhi)内部达到饱和程度(du)以(yi)便包(bao)埋。透(tou)蜡(la)时(shi)间(jian)(jian)(jian)根据(ju)组(zu)织(zhi)(zhi)(zhi)大小(xiao)而(er)定。（2）尽(jin)量(liang)保持在(zai)较(jiao)(jiao)低温度(du)中(zhong)进(jin)行，以(yi)石蜡(la)不(bu)(bu)(bu)凝(ning)固为度(du)。（3）透(tou)蜡(la)温度(du)要恒定，不(bu)(bu)(bu)可(ke)忽(hu)(hu)高(gao)忽(hu)(hu)低。（4）操作要迅速，力求在(zai)短的(de)时(shi)间(jian)(jian)(jian)内完(wan)成石蜡(la)透(tou)入过程，以(yi)免引起组(zu)织(zhi)(zhi)(zhi)变硬、变脆(cui)、收(shou)缩等。（5）注(zhu)(zhu)(zhu)意温度(du)不(bu)(bu)(bu)要过高(gao)，以(yi)免组(zu)织(zhi)(zhi)(zhi)发脆(cui)。6.染(ran)色(se)(se)(se)水(shui)洗注(zhu)(zhu)(zhu)意事项（1）染(ran)色(se)(se)(se)原理：伊(yi)(yi)红(hong)主要染(ran)细(xi)胞(bao)(bao)(bao)质(zhi)，着(zhe)色(se)(se)(se)浓(nong)淡(dan)应(ying)(ying)与(yu)苏木精(jing)染(ran)细(xi)胞(bao)(bao)(bao)核(he)的(de)浓(nong)淡(dan)相配合，如果(guo)(guo)细(xi)胞(bao)(bao)(bao)核(he)染(ran)色(se)(se)(se)较(jiao)(jiao)浓(nong)，细(xi)胞(bao)(bao)(bao)质(zhi)也应(ying)(ying)浓(nong)染(ran)，以(yi)获(huo)得鲜明的(de)对比。（2）染(ran)色(se)(se)(se)时(shi)间(jian)(jian)(jian)应(ying)(ying)根据(ju)染(ran)色(se)(se)(se)剂的(de)成熟程度(du)及室(shi)(shi)温高(gao)低，适(shi)当(dang)缩短或(huo)延长。室(shi)(shi)温高(gao)时(shi)促进(jin)染(ran)色(se)(se)(se)，染(ran)色(se)(se)(se)时(shi)间(jian)(jian)(jian)可(ke)短些，否则(ze)可(ke)适(shi)当(dang)延长时(shi)间(jian)(jian)(jian)，冬(dong)季室(shi)(shi)温低时(shi)可(ke)放(fang)入恒温箱中(zhong)染(ran)色(se)(se)(se)。（3）注(zhu)(zhu)(zhu)意流水(shui)不(bu)(bu)(bu)能过大，以(yi)防切(qie)片脱(tuo)(tuo)落。（4）如果(guo)(guo)细(xi)胞(bao)(bao)(bao)核(he)染(ran)色(se)(se)(se)较(jiao)(jiao)浅(qian)，细(xi)胞(bao)(bao)(bao)质(zhi)也应(ying)(ying)淡(dan)染(ran)。可(ke)在(zai)伊(yi)(yi)红(hong)乙醇液中(zhong)滴(di)加数滴(di)冰醋酸助(zhu)染(ran)，促使细(xi)胞(bao)(bao)(bao)质(zhi)容易(yi)着(zhe)色(se)(se)(se)，并(bing)且经乙醇脱(tuo)(tuo)水(shui)时(shi)不(bu)(bu)(bu)易(yi)褪色(se)(se)(se)。

启医疗(liao)，个体(ti)化(hua)用药贝克曼库(ku)尔(er)特(te)细(xi)胞(bao)专题--助力(li)病毒载体(ti)纯化(hua)、细(xi)胞(bao)特(te)性分(fen)(fen)析产品(pin)库(ku)仪(yi)(yi)器设备(bei)耗材(cai)试(shi)剂抗体(ti)技术(shu)服务(wu)生(sheng)物(wu)(wu)芯(xin)片(pian)芯(xin)片(pian)扫描仪(yi)(yi)|芯(xin)片(pian)点样仪(yi)(yi)|生(sheng)物(wu)(wu)芯(xin)片(pian)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|生(sheng)物(wu)(wu)芯(xin)片(pian)|其(qi)它(ta)(ta)测读(du)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)洗板机(ji)(ji)|多功能(neng)筛选(xuan)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|大型分(fen)(fen)析系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|化(hua)学发光(guang)(guang)检测仪(yi)(yi)|分(fen)(fen)光(guang)(guang)光(guang)(guang)度计(ji)|其(qi)它(ta)(ta)|光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)原子(zi)(zi)(zi)吸收光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|可见光(guang)(guang)光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|荧光(guang)(guang)光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|红外(wai)(wai)光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|近(jin)(jin)红外(wai)(wai)光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|LIBS光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|拉曼光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|紫外(wai)(wai)/可见/近(jin)(jin)红外(wai)(wai)光(guang)(guang)谱(pu)(pu)(pu)(pu)(pu)仪(yi)(yi)|其(qi)它(ta)(ta)分(fen)(fen)子(zi)(zi)(zi)生(sheng)物(wu)(wu)实(shi)验仪(yi)(yi)器电(dian)泳设备(bei)|紫外(wai)(wai)设备(bei)|普通PCR仪(yi)(yi)|定量PCR仪(yi)(yi)|数字PCR仪(yi)(yi)|DNA/有机(ji)(ji)/多肽(tai)合成|转基(ji)(ji)(ji)因仪(yi)(yi)|其(qi)它(ta)(ta)显微(wei)(wei)(wei)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)倒置显微(wei)(wei)(wei)镜|实(shi)体(ti)显微(wei)(wei)(wei)镜|生(sheng)物(wu)(wu)显微(wei)(wei)(wei)镜|电(dian)子(zi)(zi)(zi)显微(wei)(wei)(wei)镜其(qi)它(ta)(ta)显微(wei)(wei)(wei)镜|显微(wei)(wei)(wei)操纵|附件和滤光(guang)(guang)片(pian)|其(qi)它(ta)(ta)色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)液相色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|制(zhi)备(bei)液相色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|毛(mao)细(xi)管LC系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)气相色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|离子(zi)(zi)(zi)色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)检测器样品(pin)管理工(gong)具|色(se)(se)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)附件质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)飞行质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)|四极杆(gan)质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)|离子(zi)(zi)(zi)阱质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)|等离子(zi)(zi)(zi)质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)|毛(mao)细(xi)管电(dian)泳/质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)联用系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|杂(za)交(jiao)质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)|质(zhi)(zhi)谱(pu)(pu)(pu)(pu)(pu)标准品(pin)|其(qi)它(ta)(ta)实(shi)验室(shi)自(zi)动(dong)化(hua)氨基(ji)(ji)(ji)酸分(fen)(fen)析系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|蛋(dan)白(bai)纯化(hua)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|蛋(dan)白(bai)质(zhi)(zhi)翻译系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|全(quan)自(zi)动(dong)分(fen)(fen)液系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|核酸提取(qu)纯化(hua)全(quan)自(zi)动(dong)血(xue)液采(cai)样系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|基(ji)(ji)(ji)因组/蛋(dan)白(bai)组设备(bei)DNA测序(xu)仪(yi)(yi)|全(quan)基(ji)(ji)(ji)因组测序(xu)仪(yi)(yi)|基(ji)(ji)(ji)因分(fen)(fen)型系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|双向电(dian)泳系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|蛋(dan)白(bai)质(zhi)(zhi)纯化(hua)|蛋(dan)白(bai)质(zhi)(zhi)斑点切取(qu)系(xi)(xi)(xi)统(tong)(tong)(tong)(tong)(tong)(tong)|其(qi)它(ta)(ta)神经(jing)生(sheng)物(wu)(wu)学仪(yi)(yi)器动(dong)物(wu)(wu)功能(neng)检测设备(bei)|动(dong)物(wu)(wu)处理设备(bei)|。随着科技的不断(duan)进步，科研(yan)人(ren)员将能(neng)够开(kai)发出更为精(jing)确、实(shi)用的动(dong)物(wu)(wu)模型，更好(hao)地为人(ren)类健康保(bao)驾护航。

动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)在(zai)(zai)科研(yan)(yan)中(zhong)有着普(pu)遍(bian)的(de)(de)(de)应用。首(shou)先(xian)，它(ta)们可(ke)(ke)以帮助科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)深入理(li)(li)(li)解(jie)的(de)(de)(de)共同性(xing)，即不同物(wu)(wu)(wu)(wu)种(zhong)之间(jian)存在(zai)(zai)的(de)(de)(de)共有理(li)(li)(li)变化(hua)过(guo)程(cheng)(cheng)(cheng)。通(tong)过(guo)对(dui)动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)的(de)(de)(de)研(yan)(yan)究，科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)可(ke)(ke)以更(geng)清楚地了(le)(le)解(jie)的(de)(de)(de)发(fa)(fa)展过(guo)程(cheng)(cheng)(cheng)和(he)(he)(he)机制，为(wei)人(ren)(ren)(ren)(ren)类的(de)(de)(de)检(jian)查提(ti)(ti)(ti)供(gong)(gong)理(li)(li)(li)论(lun)依据。其(qi)次(ci)，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)还为(wei)新研(yan)(yan)发(fa)(fa)和(he)(he)(he)苗(miao)测试提(ti)(ti)(ti)供(gong)(gong)了(le)(le)的(de)(de)(de)平(ping)台。在(zai)(zai)研(yan)(yan)发(fa)(fa)过(guo)程(cheng)(cheng)(cheng)中(zhong)，科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)可(ke)(ke)以通(tong)过(guo)对(dui)动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)进(jin)行处理(li)(li)(li)，观(guan)察其(qi)和(he)(he)(he)副作用，为(wei)新的(de)(de)(de)临床试验提(ti)(ti)(ti)供(gong)(gong)依据。而在(zai)(zai)苗(miao)测试中(zhong)，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)则可(ke)(ke)以用来评估苗(miao)的(de)(de)(de)性(xing)和(he)(he)(he)安全性(xing)。此外，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)还为(wei)科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)提(ti)(ti)(ti)供(gong)(gong)了(le)(le)研(yan)(yan)究人(ren)(ren)(ren)(ren)类的(de)(de)(de)跨学(xue)科方法。例如，通(tong)过(guo)比较人(ren)(ren)(ren)(ren)类和(he)(he)(he)动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)的(de)(de)(de)基(ji)因组(zu)学(xue)、蛋白(bai)质(zhi)(zhi)(zhi)组(zu)学(xue)等数据，可(ke)(ke)以发(fa)(fa)现(xian)与(yu)发(fa)(fa)生(sheng)相关(guan)的(de)(de)(de)关(guan)键(jian)基(ji)因和(he)(he)(he)蛋白(bai)质(zhi)(zhi)(zhi)，从(cong)而为(wei)的(de)(de)(de)和(he)(he)(he)检(jian)查提(ti)(ti)(ti)供(gong)(gong)新的(de)(de)(de)思路。虽(sui)然动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)在(zai)(zai)科研(yan)(yan)中(zhong)发(fa)(fa)挥了(le)(le)巨(ju)大的(de)(de)(de)作用，但也存在(zai)(zai)一(yi)些挑战(zhan)。首(shou)先(xian)，由于物(wu)(wu)(wu)(wu)种(zhong)差异(yi)的(de)(de)(de)存在(zai)(zai)，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)的(de)(de)(de)表现(xian)与(yu)人(ren)(ren)(ren)(ren)类可(ke)(ke)能存在(zai)(zai)差异(yi)，因此需要谨(jin)慎使用。此外，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)的(de)(de)(de)伦理(li)(li)(li)问题也不容忽视，科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)需要在(zai)(zai)符合伦理(li)(li)(li)规定的(de)(de)(de)前(qian)(qian)提(ti)(ti)(ti)下进(jin)行相关(guan)研(yan)(yan)究。尽管(guan)存在(zai)(zai)挑战(zhan)，动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)的(de)(de)(de)发(fa)(fa)展前(qian)(qian)景(jing)仍然值得期待。随着科技的(de)(de)(de)不断进(jin)步，科研(yan)(yan)人(ren)(ren)(ren)(ren)员(yuan)(yuan)将能够开(kai)发(fa)(fa)出更(geng)为(wei)精确、实用的(de)(de)(de)动(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)型(xing)(xing)(xing)。可(ke)(ke)以发(fa)(fa)现(xian)与(yu)疾病发(fa)(fa)生(sheng)相关(guan)的(de)(de)(de)关(guan)键(jian)基(ji)因和(he)(he)(he)蛋白(bai)质(zhi)(zhi)(zhi)，从(cong)而为(wei)疾病的(de)(de)(de)预防(fang)和(he)(he)(he)检(jian)查提(ti)(ti)(ti)供(gong)(gong)新的(de)(de)(de)思路。黑龙江科研(yan)(yan)技术服(fu)务分(fen)离

动物模型的表现与人类疾病可能存在差异，因(yin)此需(xu)要谨慎(shen)使(shi)用。广西组织科(ke)研技(ji)术服务实验室

首先，预实(shi)(shi)验(yan)(yan)(yan)必(bi)须(xu)要(yao)当做正(zheng)式实(shi)(shi)验(yan)(yan)(yan)来对待（态(tai)度要(yao)放端正(zheng)，这(zhei)是(shi)(shi)必(bi)须(xu)的(de)(de)）。预实(shi)(shi)验(yan)(yan)(yan)的(de)(de)每(mei)一(yi)步都(dou)需(xu)要(yao)详(xiang)细规划，多(duo)方筹谋。不(bu)(bu)论是(shi)(shi)实(shi)(shi)验(yan)(yan)(yan)动(dong)物(wu)(wu)(wu)(wu)的(de)(de)选(xuan)择，还是(shi)(shi)检(jian)测(ce)试(shi)(shi)剂(ji)的(de)(de)购(gou)(gou)买(mai)(mai)(mai)(mai)，都(dou)尽量(liang)和(he)(he)正(zheng)式实(shi)(shi)验(yan)(yan)(yan)统一(yi)标准。建议大家(jia)先把所有(you)试(shi)(shi)剂(ji)和(he)(he)购(gou)(gou)买(mai)(mai)(mai)(mai)完(wan)毕后，再去购(gou)(gou)买(mai)(mai)(mai)(mai)实(shi)(shi)验(yan)(yan)(yan)动(dong)物(wu)(wu)(wu)(wu)。因(yin)为(wei)动(dong)物(wu)(wu)(wu)(wu)会(hui)(hui)长(zhang)胖(pang)，长(zhang)胖(pang)后给剂(ji)量(liang)就要(yao)增加，不(bu)(bu)仅多(duo)花钱，并(bing)且还容易影响实(shi)(shi)验(yan)(yan)(yan)效果。笔者曾经提前(qian)(qian)将(jiang)(jiang)实(shi)(shi)验(yan)(yan)(yan)动(dong)物(wu)(wu)(wu)(wu)买(mai)(mai)(mai)(mai)回(hui)，但因(yin)为(wei)特(te)殊原因(yin)没能购(gou)(gou)买(mai)(mai)(mai)(mai)到(dao)造模(mo)，终只能忍痛(tong)割爱将(jiang)(jiang)动(dong)物(wu)(wu)(wu)(wu)赠送(song)他人，白(bai)白(bai)亏了一(yi)笔血汗(han)钱（那批老鼠在我这(zhei)儿白(bai)吃白(bai)喝长(zhang)得太(tai)胖(pang)，没办法用作(zuo)造模(mo)）。动(dong)物(wu)(wu)(wu)(wu)及试(shi)(shi)剂(ji)购(gou)(gou)买(mai)(mai)(mai)(mai)首先需(xu)要(yao)考虑：实(shi)(shi)验(yan)(yan)(yan)动(dong)物(wu)(wu)(wu)(wu)品种、体重(zhong)、性别、周龄（通常根(gen)据既往文献报道可(ke)以获得答案）、数(shu)量(liang)（需(xu)要(yao)考虑到(dao)实(shi)(shi)验(yan)(yan)(yan)有(you)一(yi)定动(dong)物(wu)(wu)(wu)(wu)死亡率或者动(dong)物(wu)(wu)(wu)(wu)本身会(hui)(hui)打架互殴，因(yin)此需(xu)要(yao)提前(qian)(qian)购(gou)(gou)买(mai)(mai)(mai)(mai)足够的(de)(de)动(dong)物(wu)(wu)(wu)(wu)）。动(dong)物(wu)(wu)(wu)(wu)购(gou)(gou)买(mai)(mai)(mai)(mai)的(de)(de)途径(jing)、安置(zhi)的(de)(de)地点，饮食管理（一(yi)般实(shi)(shi)验(yan)(yan)(yan)室(shi)会(hui)(hui)有(you)动(dong)物(wu)(wu)(wu)(wu)房，也可(ke)以从(cong)其他地方购(gou)(gou)买(mai)(mai)(mai)(mai)），动(dong)物(wu)(wu)(wu)(wu)免证明、伦理证明需(xu)要(yao)提前(qian)(qian)准备好。实(shi)(shi)验(yan)(yan)(yan)相(xiang)关的(de)(de)试(shi)(shi)剂(ji)和(he)(he)：比如造模(mo)和(he)(he)，动(dong)物(wu)(wu)(wu)(wu)干预所需(xu)，阳性选(xuan)择及购(gou)(gou)买(mai)(mai)(mai)(mai)（有(you)些购(gou)(gou)买(mai)(mai)(mai)(mai)很(hen)困难，必(bi)须(xu)通过特(te)殊程序才能买(mai)(mai)(mai)(mai)到(dao)）。如果涉及到(dao)有(you)创操作(zuo)，要(yao)提前(qian)(qian)准备好（建议提前(qian)(qian)购(gou)(gou)买(mai)(mai)(mai)(mai)），手术(shu)。需(xu)要(yao)了解(jie)自身实(shi)(shi)验(yan)(yan)(yan)平台能完(wan)成(cheng)哪些技术(shu)。广(guang)西组织科(ke)研技术(shu)服务实(shi)(shi)验(yan)(yan)(yan)室(shi)

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