即为本(ben)发(fa)(fa)明(ming)(ming)构(gou)建(jian)的(de)(de)(de)(de)(de)(de)一种(zhong)pirb基(ji)(ji)因(yin)(yin)敲入的(de)(de)(de)(de)(de)(de)小(xiao)鼠动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)。本(ben)发(fa)(fa)明(ming)(ming)所采(cai)(cai)用第二种(zhong)技(ji)术方案的(de)(de)(de)(de)(de)(de)特(te)点(dian)还在(zai)于(yu)，步骤(zhou)1中得到(dao)grna1和(he)grna2后(hou)分别与trancrrna在(zai)25℃孵(fu)育(yu)10min形成(cheng)二级结构(gou)。步骤(zhou)3中grna1、grna2的(de)(de)(de)(de)(de)(de)浓度(du)均为2～10pmol/ul，cas9蛋白的(de)(de)(de)(de)(de)(de)注(zhu)射浓度(du)为30～100ng/μl。步骤(zhou)4中southern杂(za)交采(cai)(cai)用bamhi和(he)avrii核酸内(nei)切酶切割f1代杂(za)合子小(xiao)鼠的(de)(de)(de)(de)(de)(de)dna。本(ben)发(fa)(fa)明(ming)(ming)的(de)(de)(de)(de)(de)(de)有益(yi)效果(guo)是：本(ben)发(fa)(fa)明(ming)(ming)提供了pirb基(ji)(ji)因(yin)(yin)敲入的(de)(de)(de)(de)(de)(de)小(xiao)鼠动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)及其构(gou)建(jian)方法，本(ben)发(fa)(fa)明(ming)(ming)的(de)(de)(de)(de)(de)(de)小(xiao)鼠动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)对于(yu)pirb基(ji)(ji)因(yin)(yin)功能的(de)(de)(de)(de)(de)(de)研(yan)究和(he)在(zai)体验(yan)证提供了良好的(de)(de)(de)(de)(de)(de)基(ji)(ji)础。分离自(zi)pirb基(ji)(ji)因(yin)(yin)敲入小(xiao)鼠的(de)(de)(de)(de)(de)(de)细胞还可以用于(yu)研(yan)究pirb发(fa)(fa)挥调节作用的(de)(de)(de)(de)(de)(de)下游机制。通过pirb敲入动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)与不(bu)同类(lei)型(xing)(xing)(xing)cre小(xiao)鼠的(de)(de)(de)(de)(de)(de)杂(za)交，可以用于(yu)研(yan)究ad不(bu)同或不(bu)同细胞类(lei)型(xing)(xing)(xing)pirb的(de)(de)(de)(de)(de)(de)调节作用。欢迎致(zhi)电咨询疾病动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)建(jian)模(mo)。静安区酒精肝疾病动(dong)(dong)物(wu)(wu)模(mo)型(xing)(xing)(xing)建(jian)模(mo)

动(dong)物(wu)(wu)模型编辑添加(jia)义项名B添加(jia)义项?所(suo)属类(lei)别:经济(ji)理(li)(li)论动(dong)物(wu)(wu)疾(ji)病(bing)(bing)模型主要用于(yu)(yu)实验(yan)生理(li)(li)学、实验(yan)病(bing)(bing)理(li)(li)学和(he)(he)实验(yan)学(包括新(xin)药(yao)筛(shai)选(xuan))研究。人类(lei)疾(ji)病(bing)(bing)的发(fa)展十分复杂，以(yi)人本身作为实验(yan)对象来(lai)深入探讨疾(ji)病(bing)(bing)发(fa)生机制，推(tui)动(dong)医药(yao)学的发(fa)展来(lai)之缓慢，临床积(ji)累(lei)的经验(yan)不仅在(zai)(zai)时间(jian)和(he)(he)空间(jian)上都存在(zai)(zai)局限性，而且(qie)许多实验(yan)在(zai)(zai)道义上和(he)(he)方法上也受到限制。而借助于(yu)(yu)动(dong)物(wu)(wu)模型的间(jian)接研究，可以(yi)有意识地改变那(nei)些(xie)在(zai)(zai)自然条件下不可能或不易(yi)排除的因素，以(yi)便更(geng)准确地观察模型的实验(yan)结果并与(yu)人类(lei)疾(ji)病(bing)(bing)进行比较研究，有助于(yu)(yu)更(geng)方便、更(geng)有效地认识人类(lei)疾(ji)病(bing)(bing)的发(fa)展规律(lv)浦东新(xin)区纤(xian)维化疾(ji)病(bing)(bing)动(dong)物(wu)(wu)模型建(jian)模疾(ji)病(bing)(bing)动(dong)物(wu)(wu)模型建(jian)模怎么做？

1、自(zi)发(fa)(fa)(fa)(fa)(fa)性(xing)(xing)**动(dong)(dong)物(wu)(wu)模(mo)型:未经人为处理;自(zi)然(ran)(ran)发(fa)(fa)(fa)(fa)(fa)生(sheng);自(zi)发(fa)(fa)(fa)(fa)(fa)性(xing)(xing)**的(de)(de)总体(ti)评价--动(dong)(dong)物(wu)(wu)自(zi)发(fa)(fa)(fa)(fa)(fa)性(xing)(xing)**的(de)(de)病(bing)(bing)因(yin)(yin)(yin)往(wang)往(wang)是由动(dong)(dong)物(wu)(wu)的(de)(de)遗(yi)传(chuan)特性(xing)(xing)决定的(de)(de)与人痒的(de)(de)病(bing)(bing)因(yin)(yin)(yin)有(you)较大(da)距离。各(ge)动(dong)(dong)物(wu)(wu)**生(sheng)长(zhang)速度差异较大(da)很难在(zai)(zai)限定时间内(nei)获得大(da)量生(sheng)长(zhang)均(jun)匀的(de)(de)荷瘤动(dong)(dong)物(wu)(wu)(tumor-bearinganimals)。因(yin)(yin)(yin)此，自(zi)发(fa)(fa)(fa)(fa)(fa)性(xing)(xing)**动(dong)(dong)物(wu)(wu)模(mo)型很少(shao)在(zai)(zai)抗**药物(wu)(wu)的(de)(de)常(chang)(chang)规筛选中(zhong)(zhong)广泛(fan)应(ying)(ying)用(yong)(yong)(yong)(yong)(yong)。2、诱(you)(you)发(fa)(fa)(fa)(fa)(fa)性(xing)(xing)**动(dong)(dong)物(wu)(wu)模(mo)型:在(zai)(zai)实验(yan)条件(jian)下:使(shi)用(yong)(yong)(yong)(yong)(yong)致*物(wu)(wu)(carcinogens);诱(you)(you)发(fa)(fa)(fa)(fa)(fa)动(dong)(dong)物(wu)(wu)发(fa)(fa)(fa)(fa)(fa)生(sheng)**animalmodelofinducedtumor)指使(shi)用(yong)(yong)(yong)(yong)(yong)致*因(yin)(yin)(yin)素(carcinogens)在(zai)(zai)实验(yan)条件(jian)下诱(you)(you)发(fa)(fa)(fa)(fa)(fa)动(dong)(dong)物(wu)(wu)发(fa)(fa)(fa)(fa)(fa)生(sheng)**的(de)(de)动(dong)(dong)物(wu)(wu)模(mo)型，它是进行(xing)实验(yan)**学研(yan)究(jiu)(jiu)的(de)(de)常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)方法。常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)验(yan)证可疑致*因(yin)(yin)(yin)素的(de)(de)作用(yong)(yong)(yong)(yong)(yong)也越来越多(duo)地应(ying)(ying)用(yong)(yong)(yong)(yong)(yong)干**发(fa)(fa)(fa)(fa)(fa)生(sheng)机理的(de)(de)研(yan)究(jiu)(jiu)及(ji)防治效果的(de)(de)观察上(shang)，在(zai)(zai)**病(bing)(bing)因(yin)(yin)(yin)学、遗(yi)传(chuan)学、生(sheng)物(wu)(wu)学等(deng)方面的(de)(de)研(yan)究(jiu)(jiu)中(zhong)(zhong)有(you)重要地位。由于(yu)(yu)诱(you)(you)发(fa)(fa)(fa)(fa)(fa)因(yin)(yin)(yin)素和条件(jian)可人为控(kong)制，诱(you)(you)发(fa)(fa)(fa)(fa)(fa)率远高于(yu)(yu)自(zi)然(ran)(ran)发(fa)(fa)(fa)(fa)(fa)病(bing)(bing)率故在(zai)(zai)**实验(yan)研(yan)究(jiu)(jiu)中(zhong)(zhong)优于(yu)(yu)自(zi)发(fa)(fa)(fa)(fa)(fa)瘤。用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)诱(you)(you)发(fa)(fa)(fa)(fa)(fa)实验(yan)性(xing)(xing)**的(de)(de)动(dong)(dong)物(wu)(wu)种类很多(duo)，它们因(yin)(yin)(yin)种族不同而对(dui)相同致*因(yin)(yin)(yin)素有(you)不同的(de)(de)反(fan)应(ying)(ying)性(xing)(xing)。常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)的(de)(de)动(dong)(dong)物(wu)(wu)以哺(bu)乳(ru)动(dong)(dong)物(wu)(wu)为主(zhu)，其中(zhong)(zhong)啮齿类动(dong)(dong)物(wu)(wu)的(de)(de)使(shi)用(yong)(yong)(yong)(yong)(yong)**多(duo)，应(ying)(ying)用(yong)(yong)(yong)(yong)(yong)**广，包括各(ge)种大(da)鼠、小(xiao)鼠、际鼠等(deng)。。

诱导(dao)模型(xing)(xing)(xing)实验(yan)(yan)动物：小(xiao)鼠(shu)、豚(tun)鼠(shu)、大鼠(shu)造(zao)模试剂(ji)：烟(yan)(yan)雾(wu)、空气(qi)污染(ran)(ran)物及有(you)害(hai)气(qi)体（PM2.5、臭氧、二氧化(hua)氮）、脂(zhi)多(duo)糖(tang)(tang)、弹(dan)(dan)性蛋(dan)(dan)白(bai)酶(mei)获得(de)方式(shi)：动物全身长时间放置(zhi)在密闭容器内，暴露(lu)于烟(yan)(yan)雾(wu)、空气(qi)污染(ran)(ran)物或(huo)(huo)者有(you)害(hai)气(qi)体；气(qi)管内滴注脂(zhi)多(duo)糖(tang)(tang)或(huo)(huo)者弹(dan)(dan)性蛋(dan)(dan)白(bai)酶(mei)。模型(xing)(xing)(xing)特点：烟(yan)(yan)雾(wu)、空气(qi)污染(ran)(ran)物或(huo)(huo)者有(you)害(hai)气(qi)体诱导(dao)模型(xing)(xing)(xing)使用(yong)的(de)(de)物质、暴露(lu)的(de)(de)剂(ji)量、频次(ci)和时间不(bu)尽(jin)相同；脂(zhi)多(duo)糖(tang)(tang)造(zao)模时间短，可用(yong)来模拟急(ji)性加(jia)重反应，但不(bu)能反应慢性病(bing)(bing)变(bian)的(de)(de)过(guo)程，通常(chang)需(xu)要和其他造(zao)模方法联用(yong)。2.基(ji)因(yin)模型(xing)(xing)(xing)实验(yan)(yan)动物：α1-抗胰(yi)蛋(dan)(dan)白(bai)酶(mei)等基(ji)因(yin)敲除小(xiao)鼠(shu)获得(de)方式(shi)：直接(jie)购(gou)买模型(xing)(xing)(xing)特点：α1-抗胰(yi)蛋(dan)(dan)白(bai)酶(mei)基(ji)因(yin)敲除小(xiao)鼠(shu)是经典的(de)(de)COPD转基(ji)因(yin)模型(xing)(xing)(xing)，更准确地(di)理(li)解易感基(ji)因(yin)的(de)(de)致病(bing)(bing)机制。可以严格(ge)控制实验(yan)(yan)条件(jian)，增(zeng)强实验(yan)(yan)材料(liao)的(de)(de)可比性。

1、动(dong)(dong)物(wu)模型名称：橄(gan)榄(lan)油(you)联合酒(jiu)精(jing)(jing)致肝硬(ying)化门(men)(men)脉高压大鼠模型2、实(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)物(wu)种属：SD大鼠3、实(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)物(wu)性别：雄性4、实(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)物(wu)年龄(ling)：5周(zhou)5、实(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)物(wu)体重(zhong)(zhong)：150g-180g6、实(shi)(shi)(shi)验(yan)(yan)动(dong)(dong)物(wu)环(huan)境：SPF级1、实(shi)(shi)(shi)验(yan)(yan)方法(fa)：橄(gan)榄(lan)油(you)联合酒(jiu)精(jing)(jing)诱导。按(an)0.3mL/100g体重(zhong)(zhong)，背颈部皮下注(zhu)射50％CCl4的(de)橄(gan)榄(lan)油(you)溶(rong)液，注(zhu)射量加(jia)倍（6mL/kg体重(zhong)(zhong)），2次(ci)/周(zhou)，皮下注(zhu)射共13周(zhou)；造(zao)模前4周(zhou)为10%酒(jiu)精(jing)(jing)溶(rong)液喂养(yang)代替饮水(shui)，4周(zhou)后改为30%酒(jiu)精(jing)(jing)溶(rong)液灌(guan)胃(wei)（30%酒(jiu)精(jing)(jing)溶(rong)液灌(guan)胃(wei)1mL/100g体重(zhong)(zhong)，3次(ci)/周(zhou)）。继续正(zheng)常饲养(yang)1个月。实(shi)(shi)(shi)验(yan)(yan)结束测(ce)量门(men)(men)静脉血(xue)流量(PBF)和肠系(xi)膜(mo)上动(dong)(dong)脉血(xue)流量(SMABF)。处(chu)死，取肝脏进行(xing)组织病(bing)理学检(jian)查。2、检(jian)测(ce)标(biao)准：门(men)(men)静脉及肠系(xi)膜(mo)上动(dong)(dong)脉血(xue)流量情况与(yu)正(zheng)常对照组比较均(jun)增加(jia)，差异有(you)统计学意义肝组织病(bing)理可见肝硬(ying)化病(bing)理改变。上海东寰(huan)提供动(dong)(dong)物(wu)模型构建(jian)过程中的(de)原始实(shi)(shi)(shi)验(yan)(yan)记(ji)录及数据(ju)图(tu)片。镇江(jiang)疾病(bing)动(dong)(dong)物(wu)模型建(jian)模说明(ming)书(shu)

哪(na)里(li)可以(yi)做动(dong)物模(mo)型？静安区酒精肝(gan)疾病动(dong)物模(mo)型建模(mo)

建(jian)(jian)立肝(gan)动物模(mo)型(xing)(xing)(xing)的主要方式有(you)二(er)乙基(ji)亚(ya)硝胺（DEN）诱(you)(you)(you)发大鼠(shu)(shu)肝(gan)4-二(er)甲基(ji)氨基(ji)偶氮苯（DBA）诱(you)(you)(you)发大鼠(shu)(shu)肝(gan)。用2-乙酰氨基(ji)酸（2AAF）诱(you)(you)(you)发大鼠(shu)(shu)肝(gan)。用亚(ya)氨基(ji)偶氮甲苯（OAAT）诱(you)(you)(you)发大鼠(shu)(shu)肝(gan)。用黄(huang)曲(qu)霉(mei)素诱(you)(you)(you)发大鼠(shu)(shu)。2、胃(wei)(wei)：制备胃(wei)(wei)动物模(mo)型(xing)(xing)(xing)的主要方式有(you)①甲基(ji)胆蒽（MC）诱(you)(you)(you)发小(xiao)鼠(shu)(shu)胃(wei)(wei)。②不对(dui)称亚(ya)硝胺诱(you)(you)(you)发小(xiao)鼠(shu)(shu)胃(wei)(wei)。二(er)、消化性(xing)溃(kui)疡动物模(mo)型(xing)(xing)(xing)（animalmodelofpepticulcer）1、应(ying)激性(xing)溃(kui)疡模(mo)型(xing)(xing)(xing)是研究抗溃(kui)疡药物的常(chang)用模(mo)型(xing)(xing)(xing)。2、组(zu)胺性(xing)溃(kui)疡模(mo)型(xing)(xing)(xing)：大鼠(shu)(shu)禁食(shi)后腹部皮下(xia)注射磷酸组(zu)胺。此法也可(ke)诱(you)(you)(you)发食(shi)管、胃(wei)(wei)、十二(er)指肠等发生(sheng)溃(kui)疡。是研究溃(kui)疡发生(sheng)机制及药物的常(chang)用模(mo)型(xing)(xing)(xing)。静安区酒(jiu)精肝(gan)疾(ji)病动物模(mo)型(xing)(xing)(xing)建(jian)(jian)模(mo)

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