产品库(ku)科研资讯实(shi)(shi)(shi)验(yan)试(shi)用(yong)(yong)中心技术(shu)(shu)专题会(hui)议商城生(sheng)意(yi)通(tong)品牌求购发布产品仪器(qi)设(she)(she)备库(ku)细(xi)胞(bao)分(fen)析(xi)(xi)仪器(qi)储(chu)存保(bao)存设(she)(she)备实(shi)(shi)(shi)验(yan)室箱(xiang)体/摇床实(shi)(shi)(shi)验(yan)室安全(quan)设(she)(she)备生(sheng)物(wu)(wu)芯片(pian)(pian)3D打印仪器(qi)设(she)(she)备库(ku)生(sheng)物(wu)(wu)芯片(pian)(pian)影像系(xi)统(tong)测(ce)(ce)读系(xi)统(tong)光谱系(xi)统(tong)分(fen)子(zi)生(sheng)物(wu)(wu)实(shi)(shi)(shi)验(yan)仪器(qi)显微系(xi)统(tong)色谱系(xi)统(tong)质谱系(xi)统(tong)实(shi)(shi)(shi)验(yan)室自动(dong)化(hua)(hua)基因组/蛋(dan)白(bai)组设(she)(she)备植物(wu)(wu)生(sheng)理/动(dong)物(wu)(wu)生(sheng)理毒理/实(shi)(shi)(shi)验(yan)动(dong)物(wu)(wu)设(she)(she)备神经生(sheng)物(wu)(wu)学仪器(qi)组织学设(she)(she)备过滤装置电化(hua)(hua)学仪器(qi)细(xi)胞(bao)培(pei)养仪器(qi)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)库(ku)常(chang)用(yong)(yong)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)移(yi)液器(qi)(Pipette)PCR耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)仪器(qi)配套(tao)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)细(xi)胞(bao)/组织培(pei)养耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)分(fen)子(zi)生(sheng)物(wu)(wu)学耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)库(ku)常(chang)用(yong)(yong)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)移(yi)液器(qi)(Pipette)吸头(Tips)瓶(Bottle)瓶(Flask)管(Tube&Vial)PCR耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)微孔板色谱柱色谱介质细(xi)胞(bao)/组织培(pei)养耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)分(fen)子(zi)生(sheng)物(wu)(wu)学耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)过滤耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)仪器(qi)配套(tao)耗(hao)(hao)(hao)(hao)(hao)(hao)材(cai)试(shi)剂库(ku)细(xi)胞(bao)培(pei)养细(xi)胞(bao)干细(xi)胞(bao)免(mian)疫检测(ce)(ce)/ELISA常(chang)用(yong)(yong)生(sheng)化(hua)(hua)试(shi)剂酶试(shi)剂库(ku)生(sheng)物(wu)(wu)分(fen)子(zi)常(chang)用(yong)(yong)生(sheng)化(hua)(hua)试(shi)剂酶蛋(dan)白(bai)质/抗(kang)原/多肽cDNA及合成(cheng)纯(chun)化(hua)(hua)PCR/RT-PCR/qPCR载体及构建(jian)文库(ku)及构建(jian)克隆与(yu)(yu)表达(da)表达(da)分(fen)析(xi)(xi)核(he)酸(suan)/蛋(dan)白(bai)合成(cheng)核(he)酸(suan)分(fen)析(xi)(xi)核(he)酸(suan)检测(ce)(ce)核(he)酸(suan)纯(chun)化(hua)(hua)RNAi技术(shu)(shu)(siRNA,miRNA。动(dong)物(wu)(wu)模型(xing)的表现(xian)与(yu)(yu)人(ren)类疾病可能存在差(cha)异，因此需要谨慎使用(yong)(yong)。湖南(nan)疾病科研技术(shu)(shu)服务分(fen)离

应(ying)(ying)(ying)根(gen)据(ju)所检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)指(zhi)(zhi)标(biao)(biao)的(de)(de)(de)(de)(de)要求，需采(cai)取不(bu)同(tong)策略处理(li)(li)。在(zai)如今(jin)分(fen)(fen)(fen)子(zi)(zi)学当道(dao)的(de)(de)(de)(de)(de)现实(shi)(shi)背(bei)景下，动(dong)(dong)(dong)物实(shi)(shi)验(yan)(yan)除了对(dui)实(shi)(shi)验(yan)(yan)动(dong)(dong)(dong)物的(de)(de)(de)(de)(de)体征及生(sheng)理(li)(li)指(zhi)(zhi)标(biao)(biao)进行(xing)(xing)(xing)全的(de)(de)(de)(de)(de)监控外(wai)，各种分(fen)(fen)(fen)子(zi)(zi)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)甚至是(shi)组学检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)也(ye)开始大(da)行(xing)(xing)(xing)其道(dao)，动(dong)(dong)(dong)物实(shi)(shi)验(yan)(yan)结束之后的(de)(de)(de)(de)(de)机制研究成(cheng)为了实(shi)(shi)验(yan)(yan)的(de)(de)(de)(de)(de)重头戏。一般(ban)来说，动(dong)(dong)(dong)物实(shi)(shi)验(yan)(yan)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)对(dui)象主要包(bao)括：（1）体液(ye)中(zhong)各类(lei)(lei)因(yin)子(zi)(zi)定(ding)性(xing)及定(ding)量检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)由(you)于此(ci)类(lei)(lei)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)对(dui)象多为蛋白及各类(lei)(lei)小分(fen)(fen)(fen)子(zi)(zi)物质，因(yin)此(ci)在(zai)取样过程中(zhong)应(ying)(ying)(ying)注意(yi)(yi)防止此(ci)类(lei)(lei)物质降(jiang)解，在(zai)获(huo)取后，应(ying)(ying)(ying)及时置于温（≤-80℃）进行(xing)(xing)(xing)保(bao)(bao)存，在(zai)后续实(shi)(shi)验(yan)(yan)过程中(zhong)，也(ye)应(ying)(ying)(ying)当注意(yi)(yi)保(bao)(bao)存条件的(de)(de)(de)(de)(de)稳(wen)定(ding)性(xing)，避免反(fan)复(fu)冻融。常用(yong)的(de)(de)(de)(de)(de)方法便是(shi)酶(mei)联免吸附(fu)测(ce)(ce)(ce)(ce)(ce)(ce)定(ding)（ELISA），除此(ci)之外(wai)，可利用(yong)生(sheng)化分(fen)(fen)(fen)析(xi)仪及不(bu)同(tong)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)方法的(de)(de)(de)(de)(de)（比色(se)(se)(se)(se)法、比浊法等）方法对(dui)各类(lei)(lei)生(sheng)化指(zhi)(zhi)标(biao)(biao)及因(yin)子(zi)(zi)进行(xing)(xing)(xing)定(ding)性(xing)及定(ding)量检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)。（2）生(sheng)理(li)(li)变(bian)化及免组化检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)常用(yong)的(de)(de)(de)(de)(de)理(li)(li)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)多使用(yong)H&E染(ran)色(se)(se)(se)(se)对(dui)细(xi)(xi)(xi)(xi)胞(bao)(bao)质及细(xi)(xi)(xi)(xi)胞(bao)(bao)核(he)进行(xing)(xing)(xing)染(ran)色(se)(se)(se)(se)标(biao)(biao)记，以观察细(xi)(xi)(xi)(xi)胞(bao)(bao)变(bian)化。除此(ci)之外(wai)，许多特殊染(ran)色(se)(se)(se)(se)也(ye)在(zai)理(li)(li)生(sheng)理(li)(li)变(bian)化中(zhong)得到(dao)(dao)了应(ying)(ying)(ying)用(yong)，如利用(yong)Masson染(ran)色(se)(se)(se)(se)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)纤维(wei)化变(bian)，Nissl染(ran)色(se)(se)(se)(se)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)神经元损伤(shang)，β-半乳糖(tang)甘酶(mei)染(ran)色(se)(se)(se)(se)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)细(xi)(xi)(xi)(xi)胞(bao)(bao)衰老等。此(ci)外(wai)，还可以通过免组化技(ji)(ji)术对(dui)某些特异性(xing)标(biao)(biao)记物进行(xing)(xing)(xing)免显色(se)(se)(se)(se)检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)，达到(dao)(dao)原位定(ding)性(xing)或定(ding)量检(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)测(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)。。黑龙江外(wai)包(bao)科研技(ji)(ji)术服务实(shi)(shi)验(yan)(yan)常用(yong)于研究细(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)成(cheng)瘤能力、细(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)转移、细(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)耐(nai)药和(he)靶分(fen)(fen)(fen)子(zi)(zi)对(dui)肿瘤细(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)发展的(de)(de)(de)(de)(de)影响等等。

METTL3能够促进肺腺细(xi)(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)生长、生存和(he)(he)侵袭，但还不清(qing)楚它是否作(zuo)为m6A调节(jie)器或(huo)效(xiao)应器发(fa)挥(hui)作(zuo)用[25]。在(zai)急(ji)性髓细(xi)(xi)(xi)胞(bao)白(bai)血(xue)病（AML）患者中(zhong)(zhong)，m(6)A调控(kong)基(ji)(ji)(ji)(ji)因的(de)(de)(de)(de)(de)(de)突变(bian)或(huo)拷贝数(shu)变(bian)化(hua)(hua)与(yu)TP53突变(bian)存在(zai)密切联系，且(qie)m(6)A调控(kong)基(ji)(ji)(ji)(ji)因的(de)(de)(de)(de)(de)(de)改变(bian)与(yu)AML不良预相关[26]。此外(wai)，FTO在(zai)AML中(zhong)(zhong)高表达(da)，它通(tong)过(guo)(guo)(guo)降低mRNA转(zhuan)录本中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)m(6)水平，调节(jie)ASB2和(he)(he)RARA等靶点的(de)(de)(de)(de)(de)(de)表达(da)，增强了(le)白(bai)血(xue)病基(ji)(ji)(ji)(ji)因介(jie)导的(de)(de)(de)(de)(de)(de)细(xi)(xi)(xi)胞(bao)转(zhuan)化(hua)(hua)和(he)(he)白(bai)血(xue)病形成(cheng)(cheng)，并抑(yi)制全反式维(wei)甲酸(ATRA)诱导的(de)(de)(de)(de)(de)(de)AML细(xi)(xi)(xi)胞(bao)分(fen)(fen)化(hua)(hua)[27]。在(zai)脂肪(fang)形成(cheng)(cheng)过(guo)(guo)(guo)程中(zhong)(zhong)，FTO表达(da)与(yu)m6A水平成(cheng)(cheng)负相关，促进脂肪(fang)形成(cheng)(cheng)[3]。在(zai)胶(jiao)质细(xi)(xi)(xi)胞(bao)瘤(liu)(liu)样(yang)细(xi)(xi)(xi)胞(bao)中(zhong)(zhong)，ALKBH5通(tong)过(guo)(guo)(guo)lncRNAFOXM1介(jie)导FOXM1基(ji)(ji)(ji)(ji)因pre-mRNA上的(de)(de)(de)(de)(de)(de)m6A修(xiu)饰(shi)(shi)维(wei)持胶(jiao)质瘤(liu)(liu)细(xi)(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)成(cheng)(cheng)瘤(liu)(liu)性[28]。此外(wai)，甲基(ji)(ji)(ji)(ji)转(zhuan)移酶METTL3或(huo)METTL14的(de)(de)(de)(de)(de)(de)敲除，能够改变(bian)m6A的(de)(de)(de)(de)(de)(de)富(fu)集(ji)和(he)(he)ADAM19的(de)(de)(de)(de)(de)(de)表达(da)，极大地促进了(le)胶(jiao)质瘤(liu)(liu)细(xi)(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)生长、自我更(geng)新和(he)(he)形成(cheng)(cheng)[29]。图2m6ARNA修(xiu)饰(shi)(shi)和(he)(he)介(jie)导的(de)(de)(de)(de)(de)(de)功(gong)能[30]m6A的(de)(de)(de)(de)(de)(de)研究(jiu)方向主要是通(tong)过(guo)(guo)(guo)研究(jiu)m6A修(xiu)饰(shi)(shi)相关的(de)(de)(de)(de)(de)(de)甲基(ji)(ji)(ji)(ji)化(hua)(hua)、去甲基(ji)(ji)(ji)(ji)化(hua)(hua)酶和(he)(he)识别蛋白(bai)的(de)(de)(de)(de)(de)(de)功(gong)能，进而研究(jiu)m6A修(xiu)饰(shi)(shi)的(de)(de)(de)(de)(de)(de)生物学功(gong)能和(he)(he)作(zuo)用机制：一般通(tong)过(guo)(guo)(guo)敲除m6A酶分(fen)(fen)子(zi)，研究(jiu)下(xia)游功(gong)能基(ji)(ji)(ji)(ji)因分(fen)(fen)子(zi)的(de)(de)(de)(de)(de)(de)表达(da)和(he)(he)m6A甲基(ji)(ji)(ji)(ji)化(hua)(hua)情况，通(tong)过(guo)(guo)(guo)介(jie)导相关基(ji)(ji)(ji)(ji)因异(yi)常（可(ke)变(bian)剪(jian)切、稳(wen)定性、翻译、miRNA调控(kong)）影响细(xi)(xi)(xi)胞(bao)表型和(he)(he)功(gong)能特(te)征。

|基(ji)因(yin)组(zu)DNA扩(kuo)(kuo)增|RNA扩(kuo)(kuo)增|克(ke)隆与表达(da)克(ke)隆基(ji)因(yin)|克(ke)隆试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|克(ke)隆筛(shai)选|感(gan)受态细(xi)胞(bao)(bao)|突(tu)变(bian)转染|转化(hua)(hua)|体(ti)(ti)(ti)外转录/翻译|其(qi)它(ta)表达(da)分析Northern印迹分析|分支DNA和mRNA定(ding)(ding)(ding)量(liang)|差别基(ji)因(yin)表达(da)|反义寡核苷酸(suan)|RACE试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|SAGE试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|其(qi)它(ta)抗(kang)(kang)(kang)体(ti)(ti)(ti)蛋(dan)白(bai)(bai)A,GPLUS|抑制(zhi)/凋亡抗(kang)(kang)(kang)体(ti)(ti)(ti)|信(xin)号分子(zi)抗(kang)(kang)(kang)体(ti)(ti)(ti)结构蛋(dan)白(bai)(bai)抗(kang)(kang)(kang)体(ti)(ti)(ti)|磷酸(suan)化(hua)(hua)特异抗(kang)(kang)(kang)体(ti)(ti)(ti)|融合蛋(dan)白(bai)(bai)tag抗(kang)(kang)(kang)体(ti)(ti)(ti)非哺乳(ru)动(dong)物蛋(dan)白(bai)(bai)抗(kang)(kang)(kang)体(ti)(ti)(ti)|细(xi)胞(bao)(bao)周期蛋(dan)白(bai)(bai)抗(kang)(kang)(kang)体(ti)(ti)(ti)|转录调(diao)节蛋(dan)白(bai)(bai)抗(kang)(kang)(kang)体(ti)(ti)(ti)|抗(kang)(kang)(kang)体(ti)(ti)(ti)制(zhi)备佐剂(ji)(ji)(ji)(ji)|抗(kang)(kang)(kang)体(ti)(ti)(ti)片(pian)段|抗(kang)(kang)(kang)体(ti)(ti)(ti)同种型|抗(kang)(kang)(kang)体(ti)(ti)(ti)纯(chun)化(hua)(hua)|抗(kang)(kang)(kang)体(ti)(ti)(ti)制(zhi)备试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|其(qi)它(ta)第二抗(kang)(kang)(kang)体(ti)(ti)(ti)westernblot二抗(kang)(kang)(kang)|免疫(yi)组(zu)化(hua)(hua)二抗(kang)(kang)(kang)|其(qi)它(ta)试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)ELISA试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|免疫(yi)组(zu)化(hua)(hua)试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|放射免疫(yi)试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|westernblot试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|流式(shi)细(xi)胞(bao)(bao)试(shi)(shi)剂(ji)(ji)(ji)(ji)盒(he)(he)(he)|其(qi)它(ta)抗(kang)(kang)(kang)体(ti)(ti)(ti)相(xiang)关(guan)siRNA|重组(zu)蛋(dan)白(bai)(bai)|白(bai)(bai)蛋(dan)白(bai)(bai)|试(shi)(shi)剂(ji)(ji)(ji)(ji)|其(qi)它(ta)分子(zi)生(sheng)(sheng)(sheng)物学(xue)服务(wu)(wu)(wu)DNA提取/纯(chun)化(hua)(hua)|DNA测(ce)(ce)序|第二代高(gao)通(tong)量(liang)测(ce)(ce)序|DNA保存和活化(hua)(hua)|基(ji)因(yin)合成(cheng)|基(ji)因(yin)步行(xing)|基(ji)因(yin)组(zu)分析生(sheng)(sheng)(sheng)物信(xin)息学(xue)|SNP检(jian)测(ce)(ce)|实时定(ding)(ding)(ding)量(liang)PCR服务(wu)(wu)(wu)|文库(ku)/载体(ti)(ti)(ti)构建|寡核苷酸(suan)合成(cheng)Oligo合成(cheng)|实时PCROligo合成(cheng)|其(qi)它(ta)细(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)物学(xue)服务(wu)(wu)(wu)细(xi)胞(bao)(bao)培养|流式(shi)细(xi)胞(bao)(bao)检(jian)测(ce)(ce)|细(xi)胞(bao)(bao)毒(du)性测(ce)(ce)试(shi)(shi)|细(xi)胞(bao)(bao)因(yin)子(zi)生(sheng)(sheng)(sheng)物检(jian)测(ce)(ce)|影像服务(wu)(wu)(wu)|筛(shai)选/发育(yu)服务(wu)(wu)(wu)|其(qi)它(ta)干(gan)(gan)细(xi)胞(bao)(bao)技术服务(wu)(wu)(wu)干(gan)(gan)细(xi)胞(bao)(bao)提取|干(gan)(gan)细(xi)胞(bao)(bao)鉴(jian)定(ding)(ding)(ding)|干(gan)(gan)细(xi)胞(bao)(bao)诱(you)导分化(hua)(hua)|干(gan)(gan)细(xi)胞(bao)(bao)常规检(jian)测(ce)(ce)|干(gan)(gan)细(xi)胞(bao)(bao)扩(kuo)(kuo)增|干(gan)(gan)细(xi)胞(bao)(bao)转基(ji)因(yin)|干(gan)(gan)细(xi)胞(bao)(bao)其(qi)他相(xiang)关(guan)实验|微生(sheng)(sheng)(sheng)物学(xue)服务(wu)(wu)(wu)微生(sheng)(sheng)(sheng)物鉴(jian)定(ding)(ding)(ding)|抑菌作用测(ce)(ce)试(shi)(shi)|内测(ce)(ce)试(shi)(shi)|内。随着跨学(xue)科研究的(de)深入(ru)开展，动(dong)物疾病模(mo)型将(jiang)在(zai)未来发挥更为普(pu)遍的(de)作用，成(cheng)为生(sheng)(sheng)(sheng)命科学(xue)、医(yi)学(xue)等领域研究工具。

小(xiao)(xiao)(xiao)鼠(shu)(shu)尾(wei)尖用(yong)(yong)酒(jiu)精擦拭尾(wei)巴，引(yin)起轻微(wei)的(de)(de)(de)(de)血(xue)(xue)管(guan)扩张；用(yong)(yong)无菌(jun)手(shou)(shou)术(shu)刀(dao)、刀(dao)片(pian)或(huo)锋利(li)的(de)(de)(de)(de)剪(jian)刀(dao)，快速(su)截断小(xiao)(xiao)(xiao)鼠(shu)(shu)尾(wei)尖1-2毫米。如果需要多次(ci)，之后每(mei)次(ci)需截除(chu)2-3毫米；从尾(wei)部像(xiang)尾(wei)尖方(fang)向(xiang)按(an)摩，增加(jia)血(xue)(xue)流(liu)(liu)；用(yong)(yong)采集血(xue)(xue)液；结(jie)束后，按(an)压伤口或(huo)使用(yong)(yong)止(zhi)血(xue)(xue)剂(ji)来(lai)止(zhi)血(xue)(xue)；每(mei)次(ci)量大约可达(da)。小(xiao)(xiao)(xiao)鼠(shu)(shu)眼(yan)(yan)(yan)球(qiu)取(qu)(qu)血(xue)(xue)单手(shou)(shou)保定(ding)好小(xiao)(xiao)(xiao)鼠(shu)(shu)；必要时(shi)剪(jian)掉小(xiao)(xiao)(xiao)鼠(shu)(shu)胡须，防(fang)止(zhi)污(wu)染血(xue)(xue)液；轻压取(qu)(qu)血(xue)(xue)侧(ce)(ce)(ce)眼(yan)(yan)(yan)部皮(pi)肤(fu)(fu)，使眼(yan)(yan)(yan)球(qiu)充血(xue)(xue)突出；用(yong)(yong)弯头镊(nie)夹取(qu)(qu)眼(yan)(yan)(yan)球(qiu)并快速(su)在(zai)摘(zhai)取(qu)(qu)；同时(shi)用(yong)(yong)左(zuo)手(shou)(shou)中指(zhi)轻按(an)小(xiao)(xiao)(xiao)鼠(shu)(shu)心(xin)(xin)脏(zang)部位，以(yi)加(jia)快心(xin)(xin)脏(zang)泵血(xue)(xue)速(su)度(du)(du)；当血(xue)(xue)液流(liu)(liu)尽时(shi)，用(yong)(yong)脱臼法处(chu)死小(xiao)(xiao)(xiao)鼠(shu)(shu)；大鼠(shu)(shu)眼(yan)(yan)(yan)眶(kuang)取(qu)(qu)血(xue)(xue)先(xian)将(jiang)小(xiao)(xiao)(xiao)鼠(shu)(shu)进行麻醉(zui)，大鼠(shu)(shu)翻正反射消失时(shi)不在(zai)继续麻醉(zui)；抗(kang)凝处(chu)理(li)过(guo)的(de)(de)(de)(de)玻(bo)璃(li)毛(mao)细采样管(guan)，掰成(cheng)2-3厘(li)米长度(du)(du)；右手(shou)(shou)食指(zhi)和拇指(zhi)轻按(an)眼(yan)(yan)(yan)眶(kuang)两侧(ce)(ce)(ce)皮(pi)肤(fu)(fu)，使眼(yan)(yan)(yan)球(qiu)突出，毛(mao)细管(guan)从内侧(ce)(ce)(ce)的(de)(de)(de)(de)眼(yan)(yan)(yan)球(qiu)与眼(yan)(yan)(yan)睑的(de)(de)(de)(de)缝隙(xi)处(chu)进入，轻轻旋(xuan)转毛(mao)细管(guan)，稍微(wei)深入眼(yan)(yan)(yan)球(qiu)后上方(fang)，血(xue)(xue)液流(liu)(liu)速(su)快的(de)(de)(de)(de)时(shi)候4-5滴即可，温柔的(de)(de)(de)(de)将(jiang)毛(mao)细管(guan)取(qu)(qu)出；用(yong)(yong)棉签(qian)按(an)压大鼠(shu)(shu)眼(yan)(yan)(yan)眶(kuang)止(zhi)血(xue)(xue)，每(mei)次(ci)可取(qu)(qu)血(xue)(xue)50-100微(wei)升(sheng)，将(jiang)血(xue)(xue)液放(fang)入冰盒中保存。小(xiao)(xiao)(xiao)鼠(shu)(shu)心(xin)(xin)脏(zang)取(qu)(qu)血(xue)(xue)先(xian)将(jiang)小(xiao)(xiao)(xiao)鼠(shu)(shu)麻醉(zui)，稍靠右侧(ce)(ce)(ce)平躺在(zai)手(shou)(shou)术(shu)板上固(gu)定(ding)；左(zuo)侧(ce)(ce)(ce)第三四根肋骨间触摸心(xin)(xin)脏(zang)，跳(tiao)动明(ming)显，慢(man)慢(man)进针；针有回血(xue)(xue)停止(zhi)进针，开(kai)始取(qu)(qu)血(xue)(xue)；一次(ci)可以(yi)300到500微(wei)升(sheng)，小(xiao)(xiao)(xiao)鼠(shu)(shu)存活(huo)，放(fang)到加(jia)热垫(dian)上待小(xiao)(xiao)(xiao)鼠(shu)(shu)苏醒后放(fang)入笼中。细胞划痕(wound healing）法是简捷(jie)测定(ding)细胞迁移运动和修复能力的(de)(de)(de)(de)方(fang)法。云南疾病(bing)科(ke)研技术(shu)服务培养(yang)

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